کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1223462 967891 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of different mannan polysaccharide usage in enzyme-linked immunosorbent assay for specific antibodies determination
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Evaluation of different mannan polysaccharide usage in enzyme-linked immunosorbent assay for specific antibodies determination
چکیده انگلیسی

Polysaccharides as antigens impose the problem of the proper ELISA assay. The indirect coating using biotin-avidin or lectins are therefore used. We tried to clarify the efficiency of different approaches to this problem. Our experiments clearly demonstrate that direct coating with mannan polysaccharide on high binding ELISA plates is superior to any other combination using any intermediate protein. On the other hand, the direct coating of the normal ELISA plate with mannan was of significantly lower (P = 0.008) efficiency. The use of protein cannot be avoided in normal microplates. All previous statements are supported by experimental data in the paper.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 45, Issue 3, 5 November 2007, Pages 521–525
نویسندگان
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