کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1223487 967892 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of underivatized glucosamine in human plasma by high-performance liquid chromatography with electrochemical detection: Application to pharmacokinetic study
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of underivatized glucosamine in human plasma by high-performance liquid chromatography with electrochemical detection: Application to pharmacokinetic study
چکیده انگلیسی

A simple, rapid and sensitive high-performance liquid chromatography method with electrochemical detection was developed for the determination of glucosamine in human plasma. Plasma samples were analyzed after a simple two-step procedure of protein precipitation with subsequent dilution. The chromatographic separation was performed on a Carbopack™ column (3 mm × 150 mm) with a mobile phase consisting of water and 200 mM sodium hydroxide. Detection was performed electrochemically in a pulsed voltammetry mode. The limit of detection was 2.0 ng/ml, inter- and intra-day precision were less than 10%. The method was successfully applied to the investigation of the pharmacokinetics of glucosamine in healthy man volunteers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 50, Issue 4, 1 November 2009, Pages 671–674
نویسندگان
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