کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1224273 | 967917 | 2006 | 5 صفحه PDF | دانلود رایگان |
An HPLC–MS with electrospray ionization method for the determination of remifentanil in human plasma samples, pre-treated with SPE cartridge, has been developed and validated.Ionisation was performed by positive-ion electrospray and quadrupole filter mass spectrometer operated in the single ion-recording mode. Pre-treatment was performed using Waters Oasis® SPE cartridges. Chromatographic separation was achieved in isocratic elution using a X-Terra C8 5 μm, 150 mm × 2.1 mm i.d. column. The mobile phase consisted of a mixture of water, methanol and acetonitrile (86:10:4, v/v/v) containing 0.1% of formic acid. The method showed to be linear in the range between 0.5 and 48.0 ng/ml, the estimated LOD is 0.18 ng/ml and the LOQ is 0.5 ng/ml. The method can be used to quantify remifentanil in plasma samples taken from adult and newborn patients in a range suitable for clinical studies.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 42, Issue 3, 26 September 2006, Pages 367–371