Electrochemical study on the behavior of Morin and its interaction with DNA

Voltammetric behavior of Morin was studied in 0.1 M HAc–NaAc + 50 mM KCl (pH 3.4) solution at glassy carbon electrode (GCE) using cyclic voltammetry (CV). Morin showed an irreversible anodic peak at 0.720 V in CV which was involving two electrons and two protons. Also, the interaction of Morin with double-stranded calf thymus DNA (ctDNA) was studied by CV at GCE with an irreversible electrochemical equation. As a result of reaction with ctDNA, the voltammetric peak of Morin was a position shift and the peak current decreased. The diffusion coefficients of both free and binding Morin (Df = 1.1086 × 10−7 cm2 s−1 and Db = 8.2544 × 10−9 cm2 s−1), binding constant (K = 1.7765 × 107 cm3 mol−1), and binding site size (s = 0.8510) of the Morin–DNA complex were obtained simultaneously by non-linear fit analysis. The results demonstrate that Morin can bind to ctDNA in 0.1 M HAc–NaAc + 50 mM KCl (pH 3.4) solution and the ring B of Morin intercalates between the DNA base pairs.