کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224520 1494677 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of solid-phase microextraction combined with derivatization to the enantiomeric determination of amphetamines
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Application of solid-phase microextraction combined with derivatization to the enantiomeric determination of amphetamines
چکیده انگلیسی

The utility of combining chiral derivatization and solid-phase microextraction (SPME) for the enantiomeric analysis of primary amphetamines by liquid chromatography has been investigated. Different derivatization/extraction strategies have been evaluated and compared using the chiral reagent o-phthaldialdehyde (OPA)–N-acetyl-l-cysteine (NAC) and fibres with a Carbowax-templated resin coating. Amphetamine, norephedrine and 3,4-methylenedioxyamphetamine (MDA) were used as model compounds. On the basis of the results obtained, a new method is presented based on the derivatization of the analytes in solution followed by SPME of the OPA–NAC derivatives formed. The proposed conditions have been applied to determine the compounds of interest at low ppm levels (≤10 μg/ml) in aqueous and urine samples. Data on the linearity, reproducibility, sensitivity and selectivity are given. The utility of the described procedure for the quantification of amphetamine, norephedrine and MDA enantiomers in different kind of samples is also discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 5, 18 March 2006, Pages 1209–1217
نویسندگان
, , ,