کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1227099 968345 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of the Plasmodium vivax schizont stage proteome
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of the Plasmodium vivax schizont stage proteome
چکیده انگلیسی

With the genome of the malaria parasite Plasmodium vivax sequenced, it is important to determine the proteomes of the parasite in order to assist efforts in antigen and drug target discovery. Since a method for continuous culture of P. vivax parasite is not available, we tried to study the proteome of the erythrocytic stages using fresh parasite isolates from patients. In schizont-enriched samples, 316 proteins were confidently identified by tandem mass spectrometry. Almost 50% of the identified proteins were hypothetical, while other major categories include proteins with binding function, protein fate, protein synthesis, metabolism and cellular transport. To identify proteins that are recognized by host humoral immunity, parasite proteins were separated by two-dimensional gel electrophoresis and screened by Western blot using an immune serum from a P. vivax patient. Mass spectrometry analysis of protein spots recognized by the serum identified four potential antigens including PV24. The recombinant protein PV24 was recognized by antibodies from vivax malaria patients even during the convalescent period, indicating that PV24 could elicit long-lasting antibody responses in P. vivax patients.

Figure optionsDownload high-quality image (73 K)Download as PowerPoint slideHighlights
► Mass spectrometry analysis identified 316 proteins in P. vivax schizont stage.
► Immunoblot and mass spectrometry identified four potential antigens.
► One novel antigen could elicit long-lasting antibody response in vivax patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 9, 24 August 2011, Pages 1701–1710
نویسندگان
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