کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242174 1495785 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Designed synthesis of Graphene @titania @mesoporous silica hybrid material as size-exclusive metal oxide affinity chromatography platform for selective enrichment of endogenous phosphopeptides
ترجمه فارسی عنوان
سنتز گرافیتی گرافیتینتینا سیلیکا به عنوان فلز اندازه گیری اکسید فلزی کروماتوگرافی برای غنی سازی انتخابی فسفوپتید های اندوژن
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A G @TiO2 @mSiO2 material was synthesized to enrich endogenous phosphopeptides.
• The method has a relative low detection limit.
• The endogenous phosphopeptides can be selectively enriched from bio-samples.

In this work, a novel size-exclusive metal oxide affinity chromatography (SE-MOAC) platform was built for phosphoproteome research. The operation for preparing graphene @titania @mesoporous silica nanohybrids (denoted as G@TiO2@mSiO2) was facile and easy to conduct by grafting titania nanoparticles on polydopamine (PD)-covered graphene, following a layer of mesoporous silica was coated on the outermost layer. The G@TiO2@mSiO2 nanohybrids exhibited high sensitivity with a low detection limit of 5 amol/μL (a total amount of 1 fmol) and high selectivity for phosphopeptides at a mass ratio of phosphopeptides to non-phosphopeptides (1:1000). The size-exclusive capability of the nanohybrids were also demonstrated by enriching the phosphopeptides from the mixture of Bovine Serum Albumin (BSA), α-casein, and β-casein digests with a high mass ratio (β-casein digests: α-casein: BSA, 1:500:500), which was attributed to the large surface area and ordered mesoporous channels. In addition, the G@TiO2@mSiO2 nanohybrids were employed to capture the endogenous phosphopeptides from human serum successfully.

The graphene @titania @mesoporous silica nanohybrids (G@TiO2@mSiO2) were synthesized which could enrich endogenous phosphopeptides form complex bio-samples efficiently.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 150, 1 April 2016, Pages 296–301
نویسندگان
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