کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242200 1495785 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fit-for-purpose chromatographic method for the determination of amikacin in human plasma for the dosage control of patients
ترجمه فارسی عنوان
روش کروماتوگرافی متناسب با هدف برای تعیین آمیکاسین در پلاسمای انسانی برای کنترل دوز بیماران
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• HPLC-FLD method for the determination of amikacin (AMK) in human plasma.
• Derivatization of AMK using ortho-phtalaldehyde and N-acetyl-L-cysteine.
• Derivatives are stable at room temperature for several hours.
• Method usefulness for pharmacokinetic monitoring of patients treated with AMK.
• Method statistically comparable to the fluorescence polarization immunoassay (FPIA).

In this paper, a simple, rapid and sensitive method based on liquid chromatography with fluorimetric detection (HPLC-FLD) for the determination of amikacin (AMK) in human plasma is developed. Determination is performed by pre-column derivatization of AMK with ortho-phtalaldehyde (OPA) in presence of N-acetyl-L-cysteine (NAC) at pH 9.5 for 5 min at 80 °C. In our knowledge, this is the first time that NAC has been used in AMK derivatization. Derivatization conditions (pH, AMK/OPA/NAC molar ratios, temperature and reaction time) are optimized to obtain a single and stable, at room temperature, derivative. Separation of the derivative is achieved on a reversed phase LC column (Kromasil C18, 5 μm, 150×4.6 i.d. mm) with a mobile phase of 0.05 M phosphate buffer:acetonitrile (80:20, v/v) pumped at flow rate of 1.0 mL/min. Detection is performed using 337 and 439 nm for excitation and emission wavelengths, respectively. The method is fitted for the purpose of being a competitive alternative to the currently used method in many hospitals for AMK dosage control: fluorescence polarization immunoassay (FPIA). The method exhibits linearity in the 0.17–10 µg mL−1 concentration range with a squared correlation coefficient higher than 0.995. Trueness and intermediate precision are estimated using spiked drug free plasma samples, which fulfill current UNE-EN ISO15189:2007 accreditation schemes. Finally, for the first time, statistical comparison against the FPIA method is demonstrated using plasma samples from 31 patients under treatment with AMK.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 150, 1 April 2016, Pages 510–515
نویسندگان
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