Amperometric glucose biosensor based on biocompatible poly(dimethylaminoethyl) methacrylate microparticles

Glucose oxidase (GOx) has been immobilized within poly(dimethylamino) ethyl methacrylate (p-DMAEM) microparticles which were subsequently used as biological material in the fabrication of a glucose biosensor. The enzyme immobilization method was optimized in relation with the monomer concentration and cross-linker content. It was found that the best biosensor response corresponds to microparticles synthesized with 1.19 M monomer and 0.37% cross-linking content. Furthermore, the influence on the biosensor response of parameters such as working potential, pH, temperature, and loaded enzyme were investigated. In addition, analytical properties such as sensitivity, linear range, response time, and detection limit were determined. The biosensor was used to analyze glucose in human serum samples with satisfactory results. The useful lifetime of the biosensor is at least 520 days.