کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1243187 1495839 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Flow injection catalase activity measurement based on gold nanoparticles/carbon nanotubes modified glassy carbon electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Flow injection catalase activity measurement based on gold nanoparticles/carbon nanotubes modified glassy carbon electrode
چکیده انگلیسی

Amperometric flow injection method of hydrogen peroxide analysis was developed based on catalase enzyme (CAT) immobilization on a glassy carbon electrode (GC) modified with electrochemically deposited gold nanoparticles on a multiwalled carbon nanotubes/chitosan film.The resulting biosensor was applied to detect hydrogen peroxide with a linear response range 1.0 × 10−7–2.5 × 10−3 M with a correlation coefficient 0.998 and response time less than 10 s. The optimum conditions of film deposition such as potential applied, deposition time and pH were tested and the flow injection conditions were optimized to be: flow rate of 3 ml/min, sample volume 75 μl and saline phosphate buffer of pH 6.89.Catalase enzyme activity was successfully determined in liver homogenate samples of rats, raised under controlled dietary plan, using a flow injection analysis system involving the developed biosensor simultaneously with spectrophotometric detection, which is the common method of enzymatic assay.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 96, 15 July 2012, Pages 161–167
نویسندگان
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