Efficient voltammetric discrimination of free bilirubin from uric acid and ascorbic acid by a CVD nanographite-based microelectrode

• We used grown nanographite-based Pt microelectrode to measure free bilirubin.
• Detection of free bilirubin in the physio-pathological concentration.
• Bilirubin determination regardless of the presence of normal albumin levels.
• Voltammetric discrimination of free bilirubin, uric and ascorbic acid.
• Sensing parameters unchanged in the presence of uric and ascorbic acid.

We report a novel electrochemical sensor based on nanographite grown on platinum microelectrodes for the determination of bilirubin in the presence of normal concentrations of albumin. The albumin is a protein with an intrinsic ability to bind the bilirubin therefore reducing the concentration of the free electroactive metabolite in human fluids. In addition, the proposed device permits the discrimination of free bilirubin from two interferents, uric acid and ascorbic acid, by the separation of their oxidation peaks in voltammetry. Preliminary measurements in human serum prove that the proposed nanostructured platform can be used to detect bilirubin.

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