کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1243805 | 1495787 | 2016 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Designed synthesis of carbon-functional magnetic graphene mesoporous silica materials using polydopamine as carbon precursor for the selective enrichment of N-linked glycan Designed synthesis of carbon-functional magnetic graphene mesoporous silica materials using polydopamine as carbon precursor for the selective enrichment of N-linked glycan](/preview/png/1243805.png)
• A carbon-functionalized magnetic mesoporous material was designed and synthesized.
• The method can enrich glycans and exclude high abundance proteins.
• The material was successfully applied to enrich of N-linked glycans from complex samples.
Glycosylation, which has been confirmed to be associated with many diseases, is an important protein post-translation modification. Taking into account the low abundant of glycan, the purification of complex biological samples is considered to be very significant before mass spectrometry detection. In this work, carbon-functionalized magnetic graphene /mesoporous silica materials (C-Mag G@mSiO2 materials) with high content of carbon were designed and synthesized by using polydopamine as carbon precursor. Taking advantage of the special interaction between carbon and glycan, C-Mag G@mSiO2 materials were successfully applied to enrich N-linked glycans in different complex samples, such as standard glycoprotein digestion, the mixture of standard glycoprotein digestion, glycoprotein and non-glycoprotein, and human serum.
The carbon-functionalized magnetic graphene /mesoporous silica materials (C-Mag G@mSiO2 materials) were constructed by using polydopamine as carbon precursor, which could enrich N-linked glycans form complex sample efficiently.Figure optionsDownload as PowerPoint slide
Journal: Talanta - Volume 148, 1 February 2016, Pages 439–443