کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1246408 969754 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of fluoroquinolones in urine and serum by using high performance liquid chromatography and multiemission scan fluorimetric detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of fluoroquinolones in urine and serum by using high performance liquid chromatography and multiemission scan fluorimetric detection
چکیده انگلیسی

A high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of four fluoroquinolones. The studied compounds have been enoxacin (ENO), norfloxacin (NOR), ofloxacin (OFLO) and enrofloxacin (ENRO). An isocratic elution method, using a mixture of tetrahydrofuran (8%) and phosphate buffer (pH 3.00, 30.0 mM, 92%) as mobile phase, has been developed. Fluorimetric detection, exciting at 277 nm, and multiemission scan (407 nm for ENO, 444 nm for both NOR and ENRO and 490 nm for OFLO) has been used. Detection limits of 500, 14.7, 25.2 and 15.0 ng mL−1 for ENO, NOR, OFLO and ENRO, respectively, have been obtained. The proposed method has been satisfactorily applied to analyze NOR, OFLO and ENRO in human urine and serum samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 68, Issue 4, 15 February 2006, Pages 1215–1221
نویسندگان
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