کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1248165 1495933 2012 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functionalized inorganic nanoparticles used as labels in solid-phase immunoassays
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Functionalized inorganic nanoparticles used as labels in solid-phase immunoassays
چکیده انگلیسی

In recent years, research has focused on the development of different types of inorganic nanoparticles (NPs) to be used as labels in immunoassays instead of fluorophores or organic particles. Basically, inorganic NPs are characterized by their optimal optical properties (e.g., high photostability and high quantum yield). The inorganic NPs studied most have been silica NPs, quantum dots (QDs), noble metals, metal nanoshells and lanthanide oxides.This review presents a general overview on the progress of NP-preparation methods and NP-surface-functionalization procedures, in order to improve the sensitivity of the immunoassays involved. Other factors that must be taken into account are the type of solid-phase substrate (e.g., NP, microtiter plate, membrane and cover glass), format (i.e. direct or indirect) and the detection mode employed in the assay. Fluorescence has been used most as the detection mode. Also widely used has been time-resolved fluorescence with lanthanide ions as probes.Finally, our review of recent literature showed that most published immunoassays using inorganic NPs were applied to biological samples.


► Inorganic NPs are used as labels in immunoassays.
► Inorganic NPs show low toxicity, good bioaffinity and easy functionalization.
► Metallic nanoshells and quantum dots are promising markers for screening.
► Inorganic NPs are used in vivo analysis followed by immunohistochemistry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 31, January 2012, Pages 144–156
نویسندگان
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