کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1249063 1495894 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface-modified electrodes in the mimicry of oxidative drug metabolism
ترجمه فارسی عنوان
الکترودهای اصلاح شده سطحی در تقلید از متابولیسم اکسیداتیو دارو
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Surface-modified electrodes can mimic Phase-I metabolic reactions.
• Self-assembly monolayers support immobilization of Cytochrome P450 enzymes.
• Electropolymerized pyrrole-monosubstituted manganese tetraphenylporphyrins.
• Carbon nanomaterials are suitable for Cytochrome P450 inhibitor screening.

This review discusses different approaches that have been taken to mimic oxidative drug metabolism as executed by members of the Cytochrome P450 (CYP450) family of enzymes in humans. Non-modified electrodes can be used to produce some of the oxidative drug metabolites observed in vivo but their scope is rather limited. Modifying electrodes with simple cofactors in analogy to those observed in CYP450 but without the protein scaffold extends these possibilities and notably allows driving reactions following a CYP450-like mechanism. The review ends with approaches to immobilize CYP450s or analogs thereof on electrodes to mimic the in vivo drug metabolism fully. We discuss future perspectives with respect to the advantages and the disadvantages of each level of complexity and possible ways forward.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 70, July–August 2015, Pages 50–57
نویسندگان
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