کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1249192 1495924 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ICP-MS for absolute quantification of proteins for heteroatom-tagged, targeted proteomics
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
ICP-MS for absolute quantification of proteins for heteroatom-tagged, targeted proteomics
چکیده انگلیسی

Detection with inductively coupled plasma mass spectrometry (ICP-MS) holds great promise in tackle the challenge of absolute, reliable quantification of proteins and peptides. We identify possible niches in using ICP-MS for MS-based determination (targeted proteomics) of a limited number of proteins. We discuss research selected from the past five years to shed new light on this particular surge in the analytical potential of ICP-MS.We conclude with our assessment of research areas that could attract future analytical activity within this emerging field.


► The challenge of absolute quantification of heteroatom-tagged proteins is revisited.
► ICP-MS-derived biological information (e.g., enzymatic activity) is introduced.
► Elemental labeling: towards higher sensitivity and more.
► LA-ICP-MS as a quantitative tool for hetero-atom-tagged imaging.
► Quality control and certification of peptides using elemental MS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 40, November 2012, Pages 52–63
نویسندگان
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