کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1252393 | 1496339 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes](/preview/png/1252393.png)
The fluorescent sterol dehydroergosterol (DHE) is often used as a marker for cholesterol in cellular studies. We show by vesicle fluctuation analysis that DHE has a lower ability than cholesterol to stiffen lipid bilayers suggesting less efficient packing with phospholipid acyl chains. Despite this difference, we found by fluorescence and atomic force microscopy, that DHE induces liquid-ordered/-disordered coexistent domains in giant unilamellar vesicles (GUVs) and supported bilayers made of dipalmitoylphosphatidylcholine (DPPC), dioleylphosphatidylcholine (DOPC) and DHE or cholesterol. DHE-induced phases have a height difference of 0.9–1 nm similar as known for cholesterol-containing domains. DHE not only promotes formation of liquid–liquid immiscibility but also shows strong partition preference for the liquid-ordered phase further supporting its suitability as cholesterol probe.
Journal: Chemistry and Physics of Lipids - Volume 159, Issue 2, June 2009, Pages 114–118