کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1361001 981455 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator
چکیده انگلیسی
In chromatin, gene transcription is regulated through posttranslational modifications on the histone N-terminal tail sequences, typically an acetyl group modification on lysine residues. To realize a simple model of the gene regulation of chromatin, we designed a hydrophilic polymer grafted with histone H3 tail peptides. The polyplex formed from the polymer and DNA suppressed the gene expression effectively although the polyplex was weaker than the polyplex of poly-l-lysine and DNA. This weaker polyplex afforded the acetylation of the lysine residue of the grafted peptides by histone acetyltransferase. Subsequently, the gene expression was activated due to the relaxation of the polyplex which was brought by a cationic charge decrease in the grafted peptides. This molecular system is the first functional model of the gene regulation of the chromatin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 13, 1 July 2011, Pages 4101-4105
نویسندگان
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