Electrospun nanofibrous cellulose scaffolds with controlled microarchitecture

Introducing porosity in electrospun scaffolds is critical to improve cell penetration and nutrient diffusion for tissue engineering. Nanofibrous cellulose scaffolds were prepared by electrospinning cellulose acetate (CA) followed by saponification to regenerate cellulose. Using a computer-assisted design approach, scaffolds underwent laser ablation resulting in pores with diameters between 50 and 300 μm without damaging or modifying the surrounding scaffold area. A new mineralization method was employed in conjunction with microablation using commercial phosphate buffered saline (PBS) to soak carboxymethylcellulose surface-modified electrospun scaffolds. The resulting crystals within the scaffold on the interior of the pore had a calcium to phosphate ratio of 1.56, similar to hydroxyapatite. It was observed that porosity of the cellulose scaffolds enhanced osteoblast cell attachment at the edge of the pores, while mineralization enhanced overall cell density.

► Cellulose was transformed into a complex 3D design with controlled surface chemistry.
► Porosity was designed and fabricated in scaffold by using a CO2 direct-write laser.
► Phosphate buffered saline solution used to mineralize Ca–P on scaffold.
► Cell adhesion enhanced by scaffold mineralization.