کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1383027 1500607 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Xyloglucans from flaxseed kernel cell wall: Structural and conformational characterisation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Xyloglucans from flaxseed kernel cell wall: Structural and conformational characterisation
چکیده انگلیسی


• Xyloglucans from flaxseed kernel cell wall was studied for the first time.
• Flaxseed kernel xyloglucans have highly substituted short-branched structure.
• Flexible linear-chain conformation of flaxseed kernel xyloglucans was deduced.

The structure of ethanol precipitated fraction from 1 M KOH extracted flaxseed kernel polysaccharides (KPI-EPF) was studied for better understanding the molecular structures of flaxseed kernel cell wall polysaccharides. Based on methylation/GC–MS, NMR spectroscopy, and MALDI-TOF-MS analysis, the dominate sugar residues of KPI-EPF fraction comprised of (1,4,6)-linked-β-d-glucopyranose (24.1 mol%), terminal α-d-xylopyranose (16.2 mol%), (1,2)-α-d-linked-xylopyranose (10.7 mol%), (1,4)-β-d-linked-glucopyranose (10.7 mol%), and terminal β-d-galactopyranose (8.5 mol%). KPI-EPF was proposed as xyloglucans:Figure optionsDownload as PowerPoint slideThe substitution rate of the backbone is 69.3%; R1 could be T-α-d-Xylp-(1→, or none; R2 could be T-α-d-Xylp-(1→, T-β-d-Galp-(1 → 2)-α-d-Xylp-(1→, or T-α-l-Araf-(1 → 2)-α-d-Xylp-(1→; R3 could be T-α-d-Xylp-(1→, T-β-d-Galp-(1 → 2)-α-d-Xylp-(1→, T-α-l-Fucp-(1 → 2)-β-d-Galp-(1 → 2)-α-d-Xylp-(1→, or none. The Mw of KPI-EPF was calculated to be 1506 kDa by static light scattering (SLS). The structure-sensitive parameter (ρ) of KPI-EPF was calculated as 1.44, which confirmed the highly branched structure of extracted xyloglucans. This new findings on flaxseed kernel xyloglucans will be helpful for understanding its fermentation properties and potential applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 151, 20 October 2016, Pages 538–545
نویسندگان
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