کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1388086 1500826 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative structural characterization of 7 commercial galacto-oligosaccharide (GOS) products
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Comparative structural characterization of 7 commercial galacto-oligosaccharide (GOS) products
چکیده انگلیسی


• Seven GOS products were structurally characterized.
• Large structural diversity was observed between GOS preparations.
• 1D 1H NMR and HPAEC-PAD profiling allow structural assessment of GOS.
• Structural-reporter-group library for GOS analysis was expanded.

Many β-galactosidase enzymes convert lactose into a mixture of galacto-oligosaccharides (GOS) when incubated under the right conditions. Recently, the composition of commercial Vivinal GOS produced by Bacillus circulans β-galactosidase was studied in much detail in another study by van Leeuwen et al. As a spin-off of this study, we used the developed analytical strategy for the evaluation of 6 anonymous commercial GOS products, in comparison with Vivinal GOS. These GOS products were first subjected to HPLC-SEC, calibrated HPAEC-PAD profiling (glucose units in relation to a malto-oligosaccharide ladder), and 1D 1H NMR spectroscopy. For a more detailed analysis and support of the conclusions based on the initial analysis, the GOS products were separated into DP-pure subpools on Bio-Gel P-2 (MALDI-TOF-MS analysis), which were subjected to calibrated HPAEC-PAD profiling and 1H NMR analysis. Unidentified peaks from different GOS products, not present in Vivinal GOS, were isolated for detailed structural characterization. In this way, the differences between the various GOS products in terms of DP distribution and type of glycosidic linkages were established. A total of 13 new GOS structures were characterized, adding structural-reporter-group signals and HPAEC-PAD based glucose unit G.U. values to the analytical toolbox. The newly characterized products enhance the quality of the database with GOS structures up to DP4. The combined data provide a firm basis for the rapid profiling of the GOS products of microbial β-galactosidase enzymes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 425, 29 April 2016, Pages 48–58
نویسندگان
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