کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1388364 | 982788 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A dual role for the lex2 locus: identification of galactosyltransferase activity in non-typeable Haemophilus influenzae strains 1124 and 2019
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کلمات کلیدی
NTHiHexNAcl-glycero-d-manno-heptosePChoPETN3-deoxy-d-manno-oct-2-ulosonic acidLPSHEPMSNNeu5AcKDO - WHON-acetylneuraminic acid - اسید N-استیلون اورامینیکCapillary electrophoresis - الکتروفورزمویرگیOligosaccharide - اولیگوساکاردStructure analysis - تجزیه و تحلیل ساختارphosphoethanolamine - فسفاتانول آمینphosphocholine - فسفوسولینlipopolysaccharide - لیپوپلی ساکاریدMonophosphate - مونوفسفرهHex - هگزاHexose - هگزوزGalactosyltransferase - گالاکتوزیلتransferaseGly - گلیGlycine - گلیسین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lipopolysaccharide (LPS) of Haemophilus influenzae comprises a conserved tri-l-glycero-d-manno-heptosyl inner-core moiety (l-α-d-Hepp-(1â2)-[PEtnâ6]-l-α-d-Hepp-(1â3)-[β-d-GlcIp-(1â4)]-l-α-d-Hepp-(1â5)-α-Kdop) to which addition of β-d-Glcp to O-4 of GlcI in serotype b strains is controlled by the gene lex2B. In non-typeable H. influenzae strains 1124 and 2019, however, a β-d-Galp is linked to O-4 of GlcI. In order to test the hypothesis that the lex2 locus is involved in the expression of β-d-Galp-(1â4-β-d-Glcp-(1â from HepI, lex2B was inactivated in strains 1124 and 2019, and LPS glycoform populations from the resulting mutant strains were investigated. Detailed structural analyses using NMR techniques and electrospray-ionisation mass spectrometry (ESIMS) on O-deacylated LPS and core oligosaccharide material (OS), as well as ESIMSn on permethylated dephosphorylated OS, indicated both lex2B mutant strains to express only β-d-Glcp extensions from HepI. This provides strong evidence that Lex2B functions as a galactosyltransferase adding a β-d-Galp to O-4 of GlcI in these strains, indicating that allelic polymorphisms in the lex2B sequence direct alternative functions of the gene product.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 344, Issue 5, 31 March 2009, Pages 632-641
Journal: Carbohydrate Research - Volume 344, Issue 5, 31 March 2009, Pages 632-641
نویسندگان
Mikael K.R. Engskog, HÃ¥kan H. Yildirim, Jianjun Li, James C. Richards, Mary Deadman, Derek W. Hood, Elke K.H. Schweda,