Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period

• Osteoporosis is induced by glucocorticoids in Wistar rats during a twelve week period of time.
• FTIR results demonstrated that the composition of mandibular bone tissue was changed.
• In recovery and treatment phase the increase of mineral content was statistically significant.

The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.