کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1404390 | 1501902 | 2007 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Negatively cooperative binding of melittin to neutral phospholipid vesicles
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Negatively cooperative binding of melittin to neutral phospholipid vesicles Negatively cooperative binding of melittin to neutral phospholipid vesicles](/preview/png/1404390.png)
چکیده انگلیسی
The association of basic amphipathic peptides to neutral phospholipid membranes is investigated in terms of binding and partition models. The binding of native and modified melittin to egg-yolk phosphatidylcholine vesicles is studied by steady-state fluorescence spectroscopy. The effect of the ionic strength shows an enhancement of the association as the ionic strength increases. After correction for electrostatic effects by the Gouy-Chapman theory, the melittin binding isotherms could be described by a partition model. In terms of conventional binding mechanisms, which do not take into account electrostatic effects, this would correspond to a negative cooperativity. A plausible way in which the interaction occurs is proposed, based on the calculated Hill coefficient.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volumes 834â836, 27 May 2007, Pages 216-228
Journal: Journal of Molecular Structure - Volumes 834â836, 27 May 2007, Pages 216-228
نویسندگان
Francisco Torrens, Gloria Castellano, AgustÃn Campos, Concepción Abad,