کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1405923 1501807 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of conjugation of chloramphenicol and hemoglobin by fluorescence, circular dichroism and molecular modeling
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Analysis of conjugation of chloramphenicol and hemoglobin by fluorescence, circular dichroism and molecular modeling
چکیده انگلیسی

Chloramphenicol is a low cost, broad spectrum, highly active antibiotic, and widely used in the treatment of serious infections, including typhoid fever and other life-threatening infections of the central nervous system and respiratory tract. The purpose of the present study was to examine the conjugation of chloramphenicol with hemoglobin (Hb) and compared with albumin at molecular level, utilizing fluorescence, UV/vis absorption, circular dichroism (CD) as well as molecular modeling. Fluorescence data indicate that drug bind Hb generate quenching via static mechanism, this corroborates UV/vis absorption measurements that the ground state complex formation with an affinity of 104 M−1, and the driving forces in the Hb-drug complex are hydrophilic interactions and hydrogen bonds, as derived from computational model. The accurate binding site of drug has been identified from the analysis of fluorescence and molecular modeling, α1β2 interface of Hb was assigned to possess high-affinity for drug, which located at the β-37 Trp nearby. The structural investigation of the complexed Hb by synchronous fluorescence, UV/vis absorption, and CD observations revealed some degree of Hb structure unfolding upon complexation. Based on molecular modeling, we can draw the conclusion that the binding affinity of drug with albumin is superior, compared with Hb. These phenomena can provide salient information on the absorption, distribution, pharmacology, and toxicity of chloramphenicol and other drugs which have analogous configuration with chloramphenicol.


► Complex formation is dominance for the reduction in the β-37 Trp fluorescence.
► α1β2 interface of Hb is designated to possess high-affinity for chloramphenicol.
► Hydrophilic interactions and hydrogen bonds exist between drug and Hb.
► The Hb structure unfolds upon drug complexation.
► The binding affinity of drug with albumin is superior, compared with Hb.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 1007, 11 January 2012, Pages 81–87
نویسندگان
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