Gold nanorods–hemoglobin bio-conjugate: Spectroscopy studies

The interactions between nanostructures and biomolecules are intensely studied. Noble metal nanoparticles can modify the optical response of macromolecules. In this study, we analyzed by spectroscopic methods (UV–Vis Spectroscopy, Steady State Fluorescence, and FTIR) the bio-conjugation of horse hemoglobin with gold nanorods. Upon the formation of the gold nanorods–hemoglobin complex, the fluorescence signal increases, through Metal-enhanced fluorescence phenomenon.

► Gold nanorods were synthesized using a seed-mediated method.
► The bio-conjugate formation was studied by UV–Vis and FTIR spectroscopy.
► Gold nanorods enhance the fluorescence emission of hemoglobin through MEF.
► Fluorescence enhancement is dependent on the aspect-ratio of the nanorods.
► Major impact: potential applications in fluorescence sensors with hemoglobin.