Isolation, characterization and antioxidant activity of hyaluronic acid from marine bivalve mollusc Amussium pleuronectus (Linnaeus, 1758)

• Hyaluronic acid was purified from bivalve Amussium pleuronectus.
• HA was confirmed through spectroscopic studies & agarose gel electrophoresis.
• FTIR & NMR spectrum revealed the structure of isolated HA.
• HA showed antioxidant activities against ABTS, DPPH & Hydroxyl radicals.

The hyaluronic acid (HA) was isolated from bivalve mollusk Amussium pleuronectus and its antioxidant potential was evaluated. HA was separated from glycosaminoglycans mixture using ion-exchange chromatography (DEAE-cellulose). The net yield of HA was found to be 4.2 mg/g. The presence of HA was confirmed by agarose gel electrophoresis and further analyzed by a standard calorimetric method using Stains All. The disaccharide composition revealed the presence of uronic acid (47.7%) and N-acetyl glucosamine (35.1%). The structure of HA was characterized through FT-IR and 1H NMR spectroscopy. At 1 mg/ml, the scavenging ability of isolated HA towards ABTS, DPPH and hydroxyl radicals was 71.35, 54.42 and 63.42%, respectively. The isolated HA could be used as a potent antioxidant agent.

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