Cytotoxicity and anti-inflammatory effects of zinc ions and eugenol during setting of ZOE in immortalized human oral keratinocytes grown as three-dimensional spheroids

- Eugenol and Zn ions were released from ZOE.
- Zn ions had cytotoxicity to oral keratinocyte.
- 2D and 3D culture have different biological effects.
- Eugenol and Zn ions induced anti-inflammatory response to oral keratinocyte.