کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1420633 986376 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular weight and galloylation affect grape seed extract constituents’ ability to cross-link dentin collagen in clinically relevant time
ترجمه فارسی عنوان
وزن مولکولی و گالوویلیت بر عصاره دانه انگور تأثیر می گذارد؟ توانایی کلاژن دنتین کراس-لینک در زمان مربوطه بالینی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی

ObjectiveTo investigate the relationship between the structures of polyphenolic compounds found in grape seed extract (GSE) and their activity in cross-linking dentin collagen in clinically relevant settings.MethodsRepresentative monomeric and dimeric GSE constituents including (+)-catechin (pCT), (−)-catechin (CT), (−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-epicatechin gallate (ECG), (−)-epigallocatechin gallate (EGCG), procyanidin B2 and a pCT–pCT dimer were purchased or synthesized. GSE was separated into low (PALM) and high molecular weight (PAHM) fractions. Human molars were processed into dentin films and beams. After demineralization, 11 groups of films (n = 5) were treated for 1 min with the aforementioned reagents (1 wt% in 50/50 ethanol/water) and 1 group remained untreated. The films were studied by Fourier transform infrared spectroscopy (FTIR) followed by a quantitative mass spectroscopy-based digestion assay. Tensile properties of demineralized dentin beams were evaluated (n = 7) after treatments (2 h and 24 h) with selective GSE species that were found to protect dentin collagen from collagenase.ResultsEfficacy of GSE constituents in cross-linking dentin collagen was dependent on molecular size and galloylation. Non-galloylated species with degree of polymerization up to two, including pCT, CT, EC, EGC, procyanidin B2 and pCT–pCT dimer were not active. Galloylated species were active starting from monomeric form, including ECG, EGCG, PALM, GSE and PAHM. PALM induced the best overall improvement in tensile properties of dentin collagen.SignificanceIdentification under clinically relevant settings of structural features that contribute to GSE constituents’ efficacy in stabilizing demineralized dentin matrix has immediate impact on optimizing GSE's use in dentin bonding.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dental Materials - Volume 31, Issue 7, July 2015, Pages 814–821
نویسندگان
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