کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1421424 | 986410 | 2012 | 11 صفحه PDF | دانلود رایگان |
ObjectiveHighly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect.MethodsTo address the hypothesis that using a ZrO2/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2/HAp composite scaffolds with different ZrO2/HAp ratios, and evaluated their characteristics. In addition, porous ZrO2/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material.ResultsThe porosity of a ZrO2/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8 MPa when the ZrO2 content increased from 50 to 100 wt%. The cell adhesion and proliferation in the ZrO2/HAp scaffold was greatly improved when compared to the scaffold made with ZrO2 alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO2/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material.SignificanceWe thus showed that a porous ZrO2/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects.
Journal: Dental Materials - Volume 28, Issue 12, December 2012, Pages 1221–1231