In-vitro bio-fabrication of silver nanoparticle using Adhathoda vasica leaf extract and its anti-microbial activity

• Green synthesis of nanoparticles.
• Antimicrobial activity of synthesized nanoparticles.
• Synergetic effective study of nanoparticles and/with common antibiotic medicine penicillin.

It is well known that on treating the metallic salt solution with some plant extracts, a rapid reduction occurs leading to the formation of highly stable metal nanoparticles. Extracellular synthesis of metal nanoparticles using extracts of plants like Azadirachta indica (Neem), and Zingiber officinale (Ginger) has been reported to be successfully carried out. In this study we have developed a novel method to synthesize silver nanoparticles by mixing silver salt solution with leaf extract of Adhathoda vasica (Adulsa) without using any surfactant or external energy. By this method physiologically stable, bio-compatible Ag nanoparticles were formed which could be used for a variety of applications such as targeted drug delivery which ensures enhanced therapeutic efficacy and minimal side effects. With this method rapid synthesis of nanoparticles was observed to occur; i.e. reaction time was 1–2 h as compared to 2–4 days required by microorganisms. These nanoparticles were analyzed by various characterization techniques to reveal their morphology, chemical composition, and antimicrobial activity. TEM image of these NPs indicated the formation of spherical, non-uniform, poly-dispersed nanoparticles. A detailed study of anti-microbial activity of nanoparticles was carried out.

We have developed a novel method to synthesize silver nanoparticles by mixing silver salt solution with leaf extract of Adhathoda vasica (Adulsa) without using any surfactant or external energy. By this method physiologically stable, bio-compatible Ag nanoparticles were formed which could be used for a variety of applications. With this method rapid synthesis of nanoparticles was observed to occur; i.e. reaction time was 1–2 h as compared to 2–4 days required by microorganisms. TEM images shown reveal that there was poly-disperse spherical particles with non-uniform distribution of nanoparticles in the prepared sample. It was observed that the nanoparticles formed were of different sizes and particle size was found to be 4.74 nm, 8.17 nm, 14.23 nm and 18.98 nm and the mean size of about 11.5 nm which lies in the nano-range.Figure optionsDownload as PowerPoint slide