Increasing the number of unloading/reambulation cycles does not adversely impact body composition and lumbar bone mineral density but reduces tissue sensitivity

► Unloading significantly reduced lumbar vBMD and adipose tissue. ► Consecutive unloading cycles diminished the rate loss of lumbar vBMD and adipose tissue upon subsequent loading cycles. ► Lumbar vBMD and adipose tissue recovered post-reambulation irrespective of loss. ► Lean tissue volume was less affected by multiple unloading cycles.