کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1717187 1520029 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcription profiles of Streptococcus pneumoniae grown under different conditions of normal gravitation
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی هوافضا
پیش نمایش صفحه اول مقاله
Transcription profiles of Streptococcus pneumoniae grown under different conditions of normal gravitation
چکیده انگلیسی

High-aspect rotating vessels (HARVs) are used to study the effects low-shear modeled microgravity (LSMMG) on bacterial gene expression. LSMMG is generated by orienting HARVs with the axis of rotation perpendicular to the gravity vector while gravitational controls are oriented with the axis of rotation parallel to the gravity vector. Microarray analysis was performed on Streptococcus pneumoniae   TIGR4 grown in HARVs under three conditions (LSMMG, 1×g1×g, and static) to determine if global transcriptional activity is altered between different gravitational controls and LSMMG. Results revealed 101 differentially expressed genes under static conditions compared to 1×g1×g, 46 genes between 1×g1×g and LSMMG, and nine genes between static and LSMMG. Hierarchical cluster analysis revealed 15 genes exhibiting similar expression patterns under static conditions compared to 1×g1×g. These results indicate that rotation, in addition to low-shear forces, might contribute to bacterial adaptation to the LSMMG.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Astronautica - Volume 60, Issues 4–7, February–April 2007, Pages 433–444
نویسندگان
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