Simultaneous detection of metronidazole and chloramphenicol by differential pulse stripping voltammetry using a silver nanoparticles/sulfonate functionalized graphene modified glassy carbon electrode

• A novel and reliable AgNPs/SF-GR modified glassy carbon electrode was constructed and characterized.
• The AgNPs/SF-GR/GCE was successfully applied in the shrimp for simultaneous determination of MTZ and CAP.
• Under optimized conditions, common substances such as UA, AA, DA and ion did not interfered in the electrode performance.
• The modified electrode exhibited considerable sensitivity, stability and reproducibility.
• This fabricated electrode achieved a satisfactory level compared with other electrodes toward MTZ and CAP.

A novel silver nanoparticles/sulfonated functionalized graphene modified glassy carbon electrode (AgNPs/SF-GR/GCE) was fabricated to determine chloramphenicol and metronidazole simultaneously. Taking advantage of sulfonic group, AgNPs were successfully electrodeposited on functionalized GR immobilized on the surface of a GCE. Scanning electron microscopy and energy spectrum analysis results confirmed that AgNPs were deposited on the functionalized GR film. Compared to the bare GCE or the pristine SF-GR modified electrode, AgNPs/SF-GR/GCE exhibited excellent electroreduction towards chloramphenicol and metronidazole. In addition, the two antibacterial drugs were separated completely in 0.10 M citric acid-sodium citrate buffer (pH 4.0) by differential pulse stripping voltammetry under optimum conditions. The cathodic current was linearly related with 0.02∼20.0 μM chloramphenicol and 0.10∼20.0 μM metronidazole, with the detection limits of 0.01 μM and 0.05 μM respectively. Furthermore, AgNPs/SF-GR/GCE was applied to the simultaneous determination of chloramphenicol and metronidazole in an aquatic product.

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