کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18451 42723 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetics of immobilized cyclodextrin gluconotransferase produced by Bacillus macerans ATCC 8244
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Kinetics of immobilized cyclodextrin gluconotransferase produced by Bacillus macerans ATCC 8244
چکیده انگلیسی

The extracellular enzyme cyclodextrin gluconotransferase (CGTase, EC 2.4.1.19) was produced by Bacillus macerans ATCC 8244 at optimized conditions. The CGTase activity was found to be 495 μg β-CD/(ml min). The enzyme was partially purified by 40–60% ammonium sulfate precipitation. The enzyme was immobilized by sodium alginate beads and kinetic studies were performed. The enzymatic activity of both free and immobilized CGTase was highest at temperature 60 °C and pH 7.5. The analysis of kinetic parameters Km and Vmax was obtained by the action of CGTase on the starch with respect to β-CD. The enzyme exhibits substrate inhibition and the values are 10 and 5.5 μg starch/ml for free and immobilized enzyme, respectively. The effect of salt concentration on enzyme activity was studied and it was found that Cu2+, Fe2+, Zn2+, Co2+, Mg2+ and Hg2+ were inhibitory, however Ca2+ showed a relative increase in enzyme activity. The immobilization of the enzyme showed a high operational stability by retaining almost 75% of the initial activity after seventh use.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 39, Issue 3, 3 July 2006, Pages 507–510
نویسندگان
, ,