کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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186012 | 459606 | 2014 | 8 صفحه PDF | دانلود رایگان |
We report, a comprehensive physical and biointerfacial electrochemical characteristics of electrodeposited poly(pyrrole-co-pyrrolepropylic acid) (PPy-PPa) copolymer film on the reduced graphene oxide (RGO) sheets attached over a silane modified indium-tin-oxide coated glass, for biosensing applications. The highly specific cardiac myoglobin protein antibody, Ab-cMb, has been covalently immobilized on the copolymer film through its pendent carboxyl group by carbodiimide coupling reaction. The factor ‘n’ describing divergence of the system from ideal capacitor characteristics exhibits a low value (n = 0.59) in a constant phase element of the impedance. This low value of ‘n’ showing a porous rough microstructure of PPy-PPa film on RGO exhibiting a diffusive characteristic that has been replaced by dominant charge transfer characteristic (Ret) with n = 0.78 on biomolecular immobilization and subsequent immunoreaction. The bioelectrode exhibits a linear impedance response to human cardiac cMb marker in the range of 10 ng mL−1 to 1 μg mL−1 in phosphate buffer solution (PBS; pH 7.4) at a low frequency region of <10 Hz with a Ret sensitivity of 70.30 Ω cm2 per decade.
Journal: Electrochimica Acta - Volume 123, 20 March 2014, Pages 211–218