کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1905015 1534687 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitochondrial DNA deletions and differential mitochondrial DNA content in Rhesus monkeys: Implications for aging
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Mitochondrial DNA deletions and differential mitochondrial DNA content in Rhesus monkeys: Implications for aging
چکیده انگلیسی

The purpose of this study was to determine the relationship between mitochondrial DNA (mtDNA) deletions, mtDNA content and aging in rhesus monkeys. Using 2 sets of specific primers, we amplified an 8 kb mtDNA fragment covering a common 5.7 kb deletion and the entire 16.5 kb mitochondrial genome in the brain and buffy-coats of young and aged monkeys. We studied a total of 66 DNA samples: 39 were prepared from a buffy-coat and 27 were prepared from occipital cortex tissues. The mtDNA data were assessed using a permutation test to identify differences in mtDNA, in the different monkey groups. Using real-time RT-PCR strategy, we also assessed both mtDNA and nuclear DNA levels for young, aged and male and female monkeys. We found a 5.7 kb mtDNA deletion in 81.8% (54 of 66) of the total tested samples. In the young group of buffy-coat DNA, we found 5.7 kb deletions in 7 of 17 (41%), and in the aged group, we found 5.7 kb deletions in 12 of 22 (54%), suggesting that the prevalence of mtDNA deletions is related to age. We found decreased mRNA levels of mtDNA in aged monkeys relative to young monkeys. The increases in mtDNA deletions and mtDNA levels in aged rhesus monkeys suggest that damaged DNA accumulates as rhesus monkeys age and these altered mtDNA changes may have physiological relevance to compensate decreased mitochondrial function.


► Investigated the mtDNA deletions and mRNA expression in young, aged, male and female rhesus monkeys.
► Found increased mtDNA deletions in aged monkeys relative to young monkeys.
► mtDNA content is increased in the brains of aged monkeys, probably to compensate defective mitochondrial function.
► Discussed the compensatory aspects related to aged-dependent mitochondrial dysfunction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1822, Issue 2, February 2012, Pages 111–119
نویسندگان
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