کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1909543 | 1046729 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reactions of nitrite in erythrocyte suspensions measured by membrane inlet mass spectrometry
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کلمات کلیدی
AE14,4′-dinitrostilbene-2,2′-disulfonic acidDNDSSITSMIMSpCMBSDIDSDTPA4,4′-Diisothiocyanostilbene-2,2′-disulfonic acid - 4،4'-Diisothiocyanostilbene-2،2'-disulfonic acidErythrocytes - اریتروسیت هاDiethylenetriaminepentaacetic acid - دی اتیلنتریمین پنتا اسیدهای اسیدFree radicals - رادیکال آزادMembrane inlet mass spectrometry - طیف سنجی جرم ورودی غشاءMass spectrometry - طیف سنجی جرمیNitrite - نیتریتNitric oxide - نیتریک اکسیدHemoglobin - هموگلوبینMembrane inlet - ورودی غشاء
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The reactions of nitrite with deoxygenated human erythrocytes were examined using membrane inlet mass spectrometry to detect the accumulation of NO in an extracellular solution. In this method an inlet utilizing a silicon rubber membrane is submerged in cell suspensions and allows NO to pass from the extracellular solution into the mass spectrometer. This provides a direct, continuous, and quantitative determination of nitric oxide concentrations over long periods without the necessity of purging the suspension with inert gas. We have not observed accumulation of NO compared with controls on a physiologically relevant time scale and conclude that, within the limitations of the mass spectrometric method and our experimental conditions, erythrocytes do not generate a net efflux of NO after the addition of millimolar concentrations of nitrite. Moreover, there was no evidence at the mass spectrometer of the accumulation of a peak at mass 76 that would indicate N2O3, an intermediate that decays into NO and NO2. Inhibition of red cell membrane anion exchangers and aquaporins did not affect these processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 48, Issue 2, 15 January 2010, Pages 325-331
Journal: Free Radical Biology and Medicine - Volume 48, Issue 2, 15 January 2010, Pages 325-331
نویسندگان
Rose Mikulski, Chingkuang Tu, Erik R. Swenson, David N. Silverman,