کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910528 | 1046775 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of novel radicals from COX-catalyzed arachidonic acid peroxidation
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کلمات کلیدی
COXESRTICPOBNESR spin trapping - ESR تله اسپینcyclooxygenase - آنزیم سیکلواکسیژنازFree radicals - رادیکال آزادElectron spin resonance - رزونانس اسپین الکترونRetention time - زمان بازداریMass spectroscopy - طیف سنجی جرمیEIC - مهندسانprostaglandin - پروستاگلاندینهاhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراGas chromatography - کروماتوگرافی گازی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The peroxidation of arachidonic acid (AA) catalyzed by cyclooxygenase (COX) is a well-known free radical-mediated process that forms many bioactive products. Because of a lack of appropriate methodologies, however, no comprehensive structural evidence has been found previously for the formation of COX-mediated and AA-derived free radicals. Here we have used a combination of LC/ESR and LC/MS with a spin trap, α-[4-pyridyl-1-oxide]-N-tert-butylnitrone (POBN), to characterize the carbon-centered radicals formed from COX-catalyzed AA peroxidation in vitro, including cellular peroxidation in human prostate cancer cells (PC-3). Three types of radicals with numerous isomers were trapped by POBN as ESR-active peaks and MS-active ions of m/z 296, 448, and 548, all stemming from PGF2-type alkoxyl radicals. One of these was a novel radical centered on the carbon-carbon double bond nearest the PGF ring, caused by an unusual β-scission of PGF2-type alkoxyl radicals. The complementary nonradical product was 1-hexanol, another novel β-scission product, instead of the more common aldehyde. The characterization of these novel products formed from in vitro peroxidation provides a new mechanistic insight into COX-catalyzed AA peroxidation in cancer biology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 47, Issue 5, 1 September 2009, Pages 568-576
Journal: Free Radical Biology and Medicine - Volume 47, Issue 5, 1 September 2009, Pages 568-576
نویسندگان
Qingfeng Yu, Preeti Purwaha, Kunyi Ni, Chengwen Sun, Sanku Mallik, Steven Y. Qian,