کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910555 | 1046776 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intrastrand G-U cross-links generated by the oxidation of guanine in 5â²-d(GCU) and 5â²-r(GCU)
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کلمات کلیدی
DSSHSQCDQFSpiroiminodihydantoinDNA - DNA یا اسید دزوکسی ریبونوکلئیکCarbonate radical anion - آنیون رادیکال کربناتRNA - اسید ریبونوکلئیکUracil - اوراسیل، یوراسیلOxidation - اکسیداسیونFree radicals - رادیکال آزادsodium 2,2-dimethyl-2-silapentane-5-sulfonate - سدیم 2،2-دی متیل-2-سیلپنتان 5-سولفوناتcorrelation spectroscopy - طیف سنجی همبستگیheteronuclear single-quantum correlation - همبستگی تک کوانتومی هترو هسته ایHMBC یا Heteronuclear Multiple Bond Correlation - همبستگی پیوند چندگانه ناجورهستهheteronuclear multiple bond correlation - همبستگی چندگانه هترو هسته ایCOSY - کثیفGuanidinohydantoin - گوانیدین هیداناتینGuanine - گوانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It has been suggested that carbonate radical anions are biologically important because they may be produced during the inflammatory response. The carbonate radicals can selectively oxidize guanine in DNA and RNA by one-electron transfer mechanisms and the guanine radicals thus formed decay by diverse competing pathways with other free radicals or nucleophiles. Using a photochemical method to generate CO3â radicals in vitro, we compare the distributions of products initiated by the one-electron oxidation of guanine in the trinucleotides 5â²-r(GpCpU) and 5â²-d(GpCpU) in aqueous buffer solutions (pH 7.5). Similar distributions of stable end products identified by LC-MS/MS methods were found in both cases. The guanine oxidation products include the diastereomeric pair of spiroiminodihydantoin (Sp) and 2,5-diamino-4H-imidazolone (Iz). In addition, intrastrand cross-linked products involving covalent bonds between the G and the U bases (GâCUâ) were also found, although with different relative yields in the 2â²-deoxy- and the ribotrinucleotides. The positive-ion MS/MS spectra of the 5â²-r(GâpCpUâ) and 5â²-d(GâpCpUâ) products clearly indicate the presence of covalently linked Gâ-Uâ products that have a mass smaller by 2Â Da than the sum of the G and U bases in both types of trinucleotides. The 5â²-d(GâCUâ) cross-linked product was further characterized by 1D and 2D NMR methods that confirm its cyclic structure in which the guanine C8 atom is covalently linked to the uracil N3 atom.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 45, Issue 8, 15 October 2008, Pages 1125-1134
Journal: Free Radical Biology and Medicine - Volume 45, Issue 8, 15 October 2008, Pages 1125-1134
نویسندگان
Conor Crean, Nicholas E. Geacintov, Vladimir Shafirovich,