کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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192348 | 459739 | 2008 | 6 صفحه PDF | دانلود رایگان |
Direct electrochemistry of hemoglobin (Hb), which was immobilized on the glass carbon electrode (GCE) modified with Zn–Al layered double hydroxide (LDH) functionalized with sodium dodecylsulfonate (SDS), was investigated. The resulting electrode (Hb/LDH-SDS/GCE) gave a well-defined redox couple for HbFe(III)/Fe(II) with a formal potential of about −0.34 V (vs. AgCl/Ag) in pH 7.0 buffer. The electron-transfer rate constant was estimated to be 2.6 s−1. The Hb/LDH-SDS/GCE exhibited a remarkable electrocatalytic activity for the reduction of hydrogen peroxide (H2O2). The low calculated apparent Michaelis–Menten constant (KMapp) was 456 μM. Based on the high catalytic activity of Hb immobilized on LDH-SDS modified electrode to the reduction of H2O2, LDH functionalized with SDS is expected to have widely potential applications for development of new biosensors and biocatalysis.
Journal: Electrochimica Acta - Volume 53, Issue 24, 15 October 2008, Pages 7255–7260