کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
192845 459753 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Self-assembled monolayers-based immunosensor for detection of Escherichia coli using electrochemical impedance spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Self-assembled monolayers-based immunosensor for detection of Escherichia coli using electrochemical impedance spectroscopy
چکیده انگلیسی

An electrochemical impedance immunosensor for the detection of Escherichia coli was developed by immobilizing anti-E. coli antibodies at an Au electrode. The immobilization of antibodies at the Au electrode was carried out through a stable acyl amino ester intermediate generated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydrosuccinimide (NHS), which could condense antibodies reproducibly and densely on the self-assembled monolayer (SAM). The surface characteristics of the immunosensor before and after the binding reaction of antibodies with E. coli were characterized by atomic force microscopy (AFM). The immobilization of antibodies and the binding of E. coli cells to the electrode could increase the electro-transfer resistance, which was directly detected by electrochemical impedance spectroscopy (EIS) in the presence of Fe(CN)63−/Fe(CN)64− as a redox probe. A linear relationship between the electron-transfer resistance and the logarithmic value of E. coli concentration was found in the range of E. coli cells from 3.0 × 103 to 3.0 × 107 cfu mL−1 with the detection limit of 1.0 × 103 cfu mL−1. With preconcentration and pre-enrichment steps, it was possible to detect E. coli concentration as low as 50 cfu/mL in river water samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 53, Issue 14, 30 May 2008, Pages 4663–4668
نویسندگان
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