کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1928648 1050400 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA methyltransferase inhibitor assay system based on the HBx-induced DNA methylation of E-cadherin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
DNA methyltransferase inhibitor assay system based on the HBx-induced DNA methylation of E-cadherin
چکیده انگلیسی
We here report a simple assay system for DNA methyltransferase (DNMT) inhibitors based on the HBx-induced DNA methylation of E-cadherin. A stable cell line named G1 was generated by co-transfecting E-cadherin luciferase reporter and HBx-expression plasmid into HepG2 cells. Treatment of G1 cells with DNMT inhibitors, 5-azacytidine, 5-aza-2′-deoxycytidine, and procainamaid, dose-dependently inhibited DNA methylation of E-cadherin promoter in the reporter, resulting in up-regulation of luciferase levels and its enzyme activity. Treatment with all-trans retinoic acid that is known to inhibit DNMT expression, also induced similar effects. Our system can be useful for development of epi-drugs targeting DNA methylation in malignancies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 3, 30 August 2013, Pages 540-545
نویسندگان
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