کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1928960 | 1050437 | 2013 | 6 صفحه PDF | دانلود رایگان |
N-Acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes the formation of a β1,6-N-acetylglucosamine (GlcNAc) side chain to a core mannosyl residue in N-linked glycoproteins. Besides its direct function of producing aberrant glycoproteins, it promotes cancer progression by its involvement in the stimulation of oncoproteins. Herein, we report that GnT-V guided the transcriptional activation of membrane-type matrix metalloproteinase-1 (MT1-MMP) in cancer cells. The activated MT1-MMP expression had dual effects on cancer progression. It not only promoted proteolytic activity for cancer cells per se, but also led to the activation of MMP-2. Consequently, the activation of the two MMPs triggered by GnT-V intensified the invasive potential. A quantitative analysis using clinical tissues revealed a relatively strong correlation between GnT-V overexpression and MT1-MMP upregulation. In this study, we report for the first time that GnT-V directs cancer progression by modulating MMPs in cancer.
► Overexpression of GnT-V leads to upregulation of MT1-MMP in cancer cells.
► The GnT-V-induced upregulation of MT1-MMP promotes cancer invasion.
► MT1-MMP is upregulated in cancer tissues and the extent of upregulation increases as cancer progresses.
► MT1-MMP upregulation shows a relatively strong correlation with GnT-V overexpression in cancer tissues.
Journal: Biochemical and Biophysical Research Communications - Volume 431, Issue 4, 22 February 2013, Pages 658–663