کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1930190 | 1050491 | 2011 | 5 صفحه PDF | دانلود رایگان |

The cardiovascular system operates under demands ranging from conditions of rest to extreme stress. One mechanism of cardiac stress tolerance is action potential duration shortening driven by ATP-sensitive potassium (KATP) channels. KATP channel expression has a significant physiologic impact on action potential duration shortening and myocardial energy consumption in response to physiologic heart rate acceleration. However, the effect of reduced channel expression on action potential duration shortening in response to severe metabolic stress is yet to be established. Here, transgenic mice with myocardium-specific expression of a dominant negative KATP channel subunit were compared with littermate controls. Evaluation of KATP channel whole cell current and channel number/patch was assessed by patch clamp in isolated ventricular cardiomyocytes. Monophasic action potentials were monitored in retrogradely perfused, isolated hearts during the transition to hypoxic perfusate. An 80–85% reduction in cardiac KATP channel current density results in a similar magnitude, but significantly slower rate, of shortening of the ventricular action potential duration in response to severe hypoxia, despite no significant difference in coronary flow. Therefore, the number of functional cardiac sarcolemmal KATP channels is a critical determinant of the rate of adaptation of myocardial membrane excitability, with implications for optimization of cardiac energy consumption and consequent cardioprotection under conditions of severe metabolic stress.
► Sarcolemmal KATP channels are essential for cardiac resistance to stress.
► KATP channel activation shortens action potential duration (APD), conserving energy.
► Reduction in cardiac KATP channels does not alter magnitude of APD shortening.
► Cardiac KATP channel number defines rate of APD shortening under hypoxia.
► Sarcolemmal KATP channel expression defines cardiac energetic efficiency.
Journal: Biochemical and Biophysical Research Communications - Volume 415, Issue 4, 2 December 2011, Pages 637–641