کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933031 1050600 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-enzymatic cyclization of creatine ethyl ester to creatinine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Non-enzymatic cyclization of creatine ethyl ester to creatinine
چکیده انگلیسی

Creatine ethyl ester was incubated at 37 °C in both water and phosphate-buffered saline and the diagnostic methylene resonances in the 1H NMR spectrum were used to identify the resultant products. It was found that mild aqueous conditions result in the cyclization of creatine ethyl ester to provide inactive creatinine as the exclusive product, and this transformation becomes nearly instantaneous as the pH approaches 7.4. This study demonstrates that mild non-enzymatic conditions are sufficient for the cyclization of creatine ethyl ester into creatinine, and together with previous results obtained under enzymatic conditions suggests that there are no physiological conditions that would result in the production of creatine. It is concluded that creatine ethyl ester is a pronutrient for creatinine rather than creatine under all physiological conditions encountered during transit through the various tissues, thus no ergogenic effect is to be expected from supplementation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 388, Issue 2, 16 October 2009, Pages 252–255
نویسندگان
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