کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1933335 1050610 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Radiation-induced tetramer-to-dimer transition of Escherichia coli lactose repressor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Radiation-induced tetramer-to-dimer transition of Escherichia coli lactose repressor
چکیده انگلیسی

The wild type lactose repressor of Escherichia coli is a tetrameric protein formed by two identical dimers. They are associated via a C-terminal 4-helix bundle (called tetramerization domain) whose stability is ensured by the interaction of leucine zipper motifs. Upon in vitro γ-irradiation the repressor losses its ability to bind the operator DNA sequence due to damage of its DNA-binding domains. Using an engineered dimeric repressor for comparison, we show here that irradiation induces also the change of repressor oligomerisation state from tetramer to dimer. The splitting of the tetramer into dimers can result from the oxidation of the leucine residues of the tetramerization domain.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 2, 21 August 2009, Pages 300–304
نویسندگان
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