Hypoxia-inducible factor 1α is deregulated by the serum of rats with adjuvant-induced arthritis

Rheumatoid arthritis (RA) is known to be associated with increased risks of hypoxia-related diseases, whose progresses are critically determined by HIF-1α. The authors hypothesized that the hypoxia-related complications of RA are associated with HIF-1α deregulation by some factor(s) in RA serum. Arthritis was induced in female Lewis rats by injecting complete Freund’s adjuvant. The effects of arthritic rat serum (ARS) on hypoxic responses were investigated by incubating Hep3B cells in ARS. In the presence of ARS, HIF-1α was down-regulated and inactivated under hypoxic conditions. ARS inactivated AKT and mTOR, which led to impaired HIF-1α protein synthesis. Furthermore, insulin was found to be deficient in ARS and insulin supplementation fully recovered HIF-1α synthesis with AKT and mTOR activation. These results suggest that HIF-1α deregulation by components in serum is responsible for the RA-associated aggravation of hypoxic diseases in extra-articular tissues.