کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935089 1050658 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
H2O2 and NO scavenging by Mycobacterium leprae truncated hemoglobin O
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
H2O2 and NO scavenging by Mycobacterium leprae truncated hemoglobin O
چکیده انگلیسی
Kinetics of ferric Mycobacterium leprae truncated hemoglobin O (trHbOFe(III)) oxidation by H2O2 and of trHbOFe(IV)O reduction by NO and NO2− are reported. The value of the second-order rate constant for H2O2-mediated oxidation of trHbOFe(III) is 2.4 × 103 M−1 s−1. The value of the second-order rate constant for NO-mediated reduction of trHbOFe(IV)O is 7.8 × 106 M−1 s−1. The value of the first-order rate constant for trHbOFe(III)ONO decay to the resting form trHbOFe(III) is 2.1 × 101 s−1. The value of the second-order rate constant for NO2−-mediated reduction of trHbOFe(IV)O is 3.1 × 103 M−1 s−1. As a whole, trHbOFe(IV)O, generated upon reaction with H2O2, catalyzes NO reduction to NO2−. In turn, NO and NO2− act as antioxidants of trHbOFe(IV)O, which could be responsible for the oxidative damage of the mycobacterium. Therefore, Mycobacterium leprae trHbO could be involved in both H2O2 and NO scavenging, protecting from nitrosative and oxidative stress, and sustaining mycobacterial respiration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 373, Issue 2, 22 August 2008, Pages 197-201
نویسندگان
, , , ,