کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935595 1050670 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trans-encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Trans-encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteins
چکیده انگلیسی

A trans-packaging system for hepatitis C virus (HCV) subgenomic replicon RNAs was developed. HCV subgenomic replicon was efficiently encapsidated by the HCV structural proteins that were stably expressed in trans under the control of a mammalian promoter. Infectious HCV-like particles (HCV-LPs), established a single-round infection, were produced and released into culture medium in titers of up to 103 focus forming units/ml. Expression of NS2 protein with structural proteins (core, E1, E2, and p7) was shown to be critical for the infectivity of HCV-LPs. Anti-CD81 treatment decreased the number of infected cells, suggesting that HCV-LPs infected cells in a CD81-dependent manner. The packaging cell line should be useful both for the production of single-round infectious HCV-LPs to elucidate the mechanisms of HCV assembly, particle formation and infection to host cells, and for the development of HCV replicon-based vaccines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 371, Issue 3, 4 July 2008, Pages 446–450
نویسندگان
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